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Dawley Inc primary vascular smcs (vsmcs)
Primary Vascular Smcs (Vsmcs), supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+vascular+smcs+(vsmcs)/primary+vascular+smcs++vsmcs+/pm32212850-141-0-15
Average 90 stars, based on 1 article reviews
primary vascular smcs (vsmcs) - by Bioz Stars, 2026-09
90/100 stars

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Isolation:

Article Title: Runx2 (Runt-Related Transcription Factor 2)-Mediated Microcalcification Is a Novel Pathological Characteristic and Potential Mediator of Abdominal Aortic Aneurysm.
Article Snippet: Primary vascular SMCs (VSMCs) were isolated from thoracic aortas of 150 to 180 g male Sprague-Dawley rats by collagenase digestion as previously described.29 VSMCs were cultured in DMEM supplemented with 10% FBS at 37oC in 5% CO2.

Cell Culture:

Article Title: Runx2 (Runt-Related Transcription Factor 2)-Mediated Microcalcification Is a Novel Pathological Characteristic and Potential Mediator of Abdominal Aortic Aneurysm.
Article Snippet: Primary vascular SMCs (VSMCs) were isolated from thoracic aortas of 150 to 180 g male Sprague-Dawley rats by collagenase digestion as previously described.29 VSMCs were cultured in DMEM supplemented with 10% FBS at 37oC in 5% CO2.



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ArtMSCs did not induce SMC toxicity. ArtMSCs and Blank-MPs were incubated with SMCs for 6, 12, and 24 h, after which the SMCs were incubated with a fluorescent LIVE/DEAD assay. Green cells indicate live cells while red cells indicate dead ones. Blank-MPs caused some cytotoxicity at 12 h and 24 h, as indicated by the red cells shown by the orange arrows. The cell death control (Dead NC using diH 2 O) and live cell control (Live PC using SBM) both indicate that the LIVE/DEAD stain was functional.

Journal: Bioengineering

Article Title: Mesenchymal Stem Cell-Conditioned Media-Loaded Microparticles Enhance Acute Patency in Silk-Based Vascular Grafts

doi: 10.3390/bioengineering11090947

Figure Lengend Snippet: ArtMSCs did not induce SMC toxicity. ArtMSCs and Blank-MPs were incubated with SMCs for 6, 12, and 24 h, after which the SMCs were incubated with a fluorescent LIVE/DEAD assay. Green cells indicate live cells while red cells indicate dead ones. Blank-MPs caused some cytotoxicity at 12 h and 24 h, as indicated by the red cells shown by the orange arrows. The cell death control (Dead NC using diH 2 O) and live cell control (Live PC using SBM) both indicate that the LIVE/DEAD stain was functional.

Article Snippet: Human aortic vascular smooth muscle cells (SMCs) either from ATCC (for toxicity assessment, Manassas, VA, PCS-100-012) or Cell Applications (for proliferation assays, 354-05a, San Diego, CA, USA) were cultured in supplemented basal media (SBM) (311K-500, Cell Applications Inc.).

Techniques: Incubation, Live Dead Assay, Control, Staining, Functional Assay